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Rabbit anti-SRBC antiserum (Rockland, Gilbertsville, PA) for 30 min at room temperature, then the erythrocytes were washed, plated on monolayer of macrophages at a ratio of 20:1. Fcreceptor-dependent phagocytosis of IgG-coated bovine erythrocytes by ZK cell lines was performed as described by Mbawuike IN et al. [40] with some modification. Briefly, ZK cells were plated at 1 ?106 cells/well in a 6-
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Bs: pre-incubate the cells with 0.5 g of mAb 2.4G2 (anti-mouse CD16/CD32, to block binding of FcR) per million cells for 5?0 minutes on ice prior to staining, then add 0.25 g/ml of FITC-labeled anti-mouse F4/80 or FITC-labeled anti-mouse CD11b to the cells in each tube, mix gently and incubate for 30 min in the dark. All staining procedures were carried out at 4 . Cells were then washed 3 times an
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Breast tumors, colorectal cancer cells often show an overlapping growth pattern in the examination specimen making FISH analysis less reproducible and harder to interpret. The CISH technique seems able to overcame this potentially confounding factor as it offers the opportunity to morphologically identify overlapping tumor cells, which can be consequently excluded from the analysis. In our series
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Ested that pulmonary inflammation mediated by particle-induced oxidative stress may play an important role [13,14]. Generation of reactive oxygen and nitrogen species (ROS/RNS) either directly or through activation of phagocytes can cause oxidative damage to DNA leading to initiation of cancer [14]. Additionally, ROS may potentiate tumor development by stimulating production of pro-inflammatory me
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Itada M, Kitagawa H, Igarashi K, Hirose S, Kanakubo Y: Polyamine lowered the hepatic lipid peroxide level in rats. Res Commun Chem Pathol Pharmacol 1988, 62:235-249. Merentie M, Uimari A, Pietil?M, Sinervirta R, Kein en TA, Veps nen J, Khomutov A, Grigorenko N, Herzig KH, J ne J, Alhonen L: Oxidative stress and inflammation in the pathogenesis of activated polyamine catabolism-induced acute pancr
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Ormation were obtained. RNA was extracted from plasma samples, reverse transcribed and PCR amplified as described previously [12] using subtype non-specific HIV-1 primers for HIV-1 full length gag [12] and nef [13] genes, and sequenced. Sequenced fragments were assembled using ChromasPro. Full length gag and nef sequences were generated and aligned using MUSCLE with manual editing in MEGA5, togeth
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C No tumors were detected in B6 mice in any of the experimental groups examined (data not shown). d A significant strain effect was detected for tumor size (mm) between A/J (0.72 ?0.012) and BALB mice (0.59 ?0.054) (P
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Rolina). Time- and strain- dependent changes in BALF protein, cellularity, chemokines, nuclear transcription factor activity, protein densitometry, and tumor multiplicity/size were analyzed using an analysis of variance (ANOVA). When statistical differences were detected (P