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Of PMNsFigure 1 Experimental protocols. A.) V2O5 was evaluated as a tumor promoter using a two-stage carcinogenesis model. MCA suspended in corn oil (10 g/g) was administered to initiate carcinogenesis followed by 5 weekly aspirations of V2O5 (4 mg/kg, promoter) or PBS. B.) A/J, BALB, and B6 mice were exposed to 4 weekly aspirations of V2O5 and sacrificed at select time points to assess pulmonary
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Rolina). Time- and strain- dependent changes in BALF protein, cellularity, chemokines, nuclear transcription factor activity, protein densitometry, and tumor multiplicity/size were analyzed using an analysis of variance (ANOVA). When statistical differences were detected (P
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Exposed acutely to V2O5. Coughing, wheezing, chest pain, bronchitis, and asthma-like symptoms as well as impaired lung function occurred in humans exposed to high levels of V2O5-containing dust [22-25]. In primates, inhalation of V2O5 particles increased bronchoaveolar polymorphonuclear neutrophils (PMNs) and impaired pulmonary function [26], and in rodents, inhalation or intratracheal administrat
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S from MARCO-/- and SR-AI/II-/- mice, they demonstrate marked decreased binding and phagocytosis of these particles compared to primary AMs from wild type mice (Fig. 6A and Fig. 7A and Fig. 7B). The results confirm that MARCO and SR-AI/II receptors on alveolar macrophages play critical roles in uptake of unopsonized environmental particles and bacteria. Moreover, the reduced, but still demonstrabl
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Ing time (mean generation time = mgt) was calculated according the formula: N = N02T/mgt. On the average, doubling time of ZK cell lines is between 12 h to 16 h in RPMI complete medium (Fig. 2). Like their parental primary AMs isolated from the MS-/- mice, all of the cell lines are adherent but trypsin-sensitive for passage. Morphology Light microscopic examination of Diff Quik, a modified Wright-
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Of PMNsFigure 1 Experimental protocols. A.) V2O5 was evaluated as a tumor promoter using a two-stage carcinogenesis model. MCA suspended in corn oil (10 g/g) was administered to initiate carcinogenesis followed by 5 weekly aspirations of V2O5 (4 mg/kg, promoter) or PBS. B.) A/J, BALB, and B6 mice were exposed to 4 weekly aspirations of V2O5 and sacrificed at select time points to assess pulmonary
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Ark cycle. Animals were given standard laboratory chow (Teklan foods, Indianapolis, IN) and spring water ad libitum and were assessed daily for health status. All mice were allowed one week to acclimatize prior to treatment. Animal use was conducted in AAALAC-accredited facilities and in accordance with the regulatory guidelines of the Michigan State University All University Committee on Animal U
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And 1.2-fold in B6 mice.Rondini et al. Particle and Fibre Toxicology 2010, 7:9 http://www.particleandfibretoxicology.com/content/7/1/Page 6 ofFigure 2 A/J and BALB are susceptible to pulmonary inflammation and hyperpermeability in response to sub-chronic V2O5. A/J, BALB, and B6 mice were exposed to 4 weekly doses of V2O5 (4 mg/kg) by aspiration and sacrificed 0.25, 1, 3, 6, 21 days after the last