Ch lobe were captured under the microscope at magnification ?5 prior to being viewed with a Zeiss epifluorescence microscope. Images were obtained and digitized (TIFF format; with a resolution of 8 pixels/m). H E stained color was picked by Biopix software (Version 2.1.3; Biopix, www.biopix.se) according to the hue, saturation and brightness of theNallapalli et al. Molecular Cancer 2012, 11:50 htt