N1/Xho1 site of this vector. The PCR products (pGL3-MMP-9WT) were confirmed by their size, as determined by electrophoresis, and by DNA sequencing. Additionally, the introduction of a mismatched primer mutation into the AP-1 to generate pGL3-MMP-9AP1 was performed, using the following (forward) primer: AP-1: 5'-GCAGGAGAGGAAGCTGAGTTGAAGA CA-3'. MMP-9-luc plasmid was transfected into RBA-1 cells. Al